Forager-Fast Plasmid Subcloning Kit

Many plasmids that work in E. coli are compatible with Forager-1. However, some plasmid origins are not efficiently recognized and not every selection marker works identically. Confirmed origins include pMB1, ColE1, pUC, and RK2, and selection with carbenicillin, kanamycin, and chloramphenicol markers work well. We are always testing and optimizing these here at Forage, check the latest manual for details.

We generally do not recommend a recovery between transformation and plating. In our proprietary media, Forager-1 is competent and metabolically active, so transformation and selection marker expression happens in a single step. Since Forager-1 doubles rapidly in rich media, replication during a prolonged recovery is likely to inflate the apparent transformation efficiency. However, for some difficult plasmids, a brief recovery (~30 minutes) may be beneficial.

V-nat is known to have poor cold tolerance. We know its not ideal, but plates can be stored at room temperature for several days, and once a glycerol stock is made fresh colonies are always just 6 hours away!

Yes! We would be happy to offer discounted pricing to non-profit academic and educational users. Email us at customerservice@forageevo.com for details.

Your purchase of the Forager-Fast Plasmid Subcloning Kit currently includes a limited license for direct plasmid cloning and propagation purposes only. However, in most cases we are willing to granting expanded rights for other uses like protein expression upon request. This restriction is intended for us to keep track of where and how Forager-1 is used, but we want Foragers like yourself to explore what it can do so just ask! See our terms and conditions for details, or email us at customerservice@forageevo.com.